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Minimum list of equipment (excluding laminar cabinets) for the detection of nucleic acids of infectious diseases by polymerase chain reaction in real time (PCR)

published 28 June 2022 11:20

Currently approved support: 

Discussions with Government of Canada are underway with the participation of management and technical specialists of the State Service of Ukraine on Food Safety and Consumer Production to support the establishment of PCR laboratories, information is being exchanged.

Description and Calculations

For the creation of new export routes for agricultural products, it became necessary to purchase the PCR equipment (specifically for timely diagnostic, monitoring of animal health status for Ukraine, further certification for export), animal health surveillance is the part of trade conditions in accordance with safe trade rules of OIE.

The total need is 6 units of the PCR equipped laboratories, including future ones on currently occupied territories. You can find the detailed list of equipment necessary for functioning of one unit.

І. List of equipment by work areas

1. Room for preparation of samples for nucleic acid isolation

No.

Name of equipment

Quantity, items

1

Homogenizer for pathological material

1

2

High-speed centrifuge (13 thousand or more rpm) with cooling for test tubes with a volume of 1.5-2 ml

1

3

Mini centrifuge shaker for test tubes with a volume of 1.5-2 ml

1

4

A set of single-channel dispensers with variable volumes (0.5-10 μl;

2-20 μl, 10-100 μl; 20-200 μl; 100-1000 μl)

1

5

Refrigerator with freezer (-20 °C) for short-term storage of pathological material

1

6

Freezer (-80 °C) (for long-term storage of pathological material)

1

7

Dry oven (with a temperature of 180 °C) for sterilization of tools

1

2. Nucleic acid isolation room

No.

Name of equipment

Quantity, items

1

Thermoshaker (with variable temperature and shaking frequency) for test tubes with a volume of 1.5-2 ml

1

2

High-speed centrifuge (13 thousand or more rpm) with cooling for 1.5-2 ml test tubes

1

3

Mini centrifuge shaker for test tubes with a volume of 1.5-2 ml

1

4

Set of single-channel dispensers with variable volumes (0.5-10 μl; 2-20 μl, 10-100 μl; 20-200 μl; 100-1000 μl)

1

5

Refrigerator with freezer (-20 °C) (for storage of components of diagnostic kits)

1

6

Laboratory suction pump

1

3. Room for preparation of reaction mixtures

No.

Name of equipment

Quantity, items

1

PCR box (one for the preparation of the mastermix, the other for the introduction of nucleic acid test samples into the reaction mixture)

2

2

Mini centrifuge shaker for test tubes with a volume of 1.5-2 ml

2

3

Mini centrifuge shaker for striped (8 pcs) tubes with a volume of 0.1-0.2 ml

1

4

Set of single-channel dispensers with variable volumes (0.5-10 μl; 2-20 μl, 10-100 μl; 20-200 μl; 100-1000 μl)

2

5

Refrigerator with freezer (-20 °C) (for short-term storage of samples of isolated nucleic acids)

1

6

Freezer (-20 °C) (volume 200 l) for storage of diagnostic kits

1

4. PCR room

No.

Name of equipment

Quantity, items

1

Amplifier for real-time PCR (with laptop)

1


ІІ. Generalized list of equipment

No.

Name of equipment

Quantity, items

1

Homogenizer for pathological material

1

2

High-speed centrifuge (13 thousand or more rpm) with cooling for test tubes with a volume of 1.5-2 ml

2

3

Mini centrifuge shaker for test tubes with a volume of 1.5-2 ml

4

4

A set of single-channel dispensers with variable volumes (0.5-10 μl;

2-20 μl, 10-100 μl; 20-200 μl; 100-1000 μl)

4

5

Refrigerator with freezer (-20 °C)

3

6

Freezer (-80 °C)

1

7

Dry oven (with a temperature of 180 °C)

1

8

Thermoshaker (with variable temperature and shaking frequency) for test tubes with a volume of 1.5-2 ml

1

9

Laboratory suction pump

1

10

PCR box

2

11

Mini centrifuge shaker for striped (8 pcs) tubes with a volume of 0.1-0.2 ml

1

12

Freezer (-20 °C) (volume 200 l) for storage of diagnostic kits

1

13

Amplifier for real-time PCR (with laptop)

1


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